کاربرد نانوالیاف الکتروریسی‌شده در مهندسی بافت: داربست‌هایی با رهایش آهسته عامل های رشد

نوع مقاله : تالیفی

نویسندگان

1 تربیت مدرس

2 دانشگاه تربیت مدرس

چکیده

ﻣﻬﻨﺪﺳﯽ ﺑﺎﻓﺖ، ﯾﮏ روﯾﮑﺮد ﻧﻮﯾﻦ درﻣﺎﻧﯽ اﺳﺖ ﮐﻪ در آن ﺳﻪ ﻋﺎﻣﻞ ﺳﻠﻮل، ﻣﺤﺮک­ﻫﺎی ﻣﻮﻟﮑﻮﻟﯽ و دارﺑﺴﺖ ﻧﻘﺶ مهمی دارﻧﺪ. اﻣﺮوزه ﭘﮋوﻫﺸﮕﺮان ﻣﻬﻨﺪﺳﯽ ﺑﺎﻓﺖ، ﺑﺮای درﻣﺎن ﺑﯿﻤﺎری­ﻫﺎی ﻣﺨﺘﻠﻒ و ﺑﺎزﺗﻮﻟﯿﺪ و جای­گذاری انواع ﺑﺎﻓﺖ­ﻫﺎی ﺑﺪن، از دارﺑﺴﺖ­ﻫﺎی ﻧﺎﻧﻮﻟﯿﻔﯽ به­ منظور ﺗﻘﻠﯿﺪ از ساختار ﺑﺎﻓﺖ ﻃﺒﯿﻌﯽ و به­ عنوان راه­ حلی برای پیشرفت و توسعه داربست­ های مهندسی بافت، اﺳﺘﻔﺎده ﻣﯽ­ﮐﻨﻨﺪ. ﺑدﯾﻦ ﻣﻨﻈﻮر، از میان تمام روش ­های موجود، معمولا اﻟﮑﺘﺮورﯾﺴﯽ انتخاب می­ شود که روش آﺳﺎن و ﻣﻘﺮون ﺑﻪ­ﺻﺮﻓﻪ­ای ﺑﺮای ﺗﻮﻟﯿﺪ اﯾﻦ ﺳﺎزه­ﻫﺎﺳﺖ. زیرا، تنوع و سازه­ های دستگاهی این فرایند، ﺗﻮﻟﯿﺪ ﻣﯿﮑﺮو-ﻧﺎﻧﻮاﻟﯿﺎف را ﺑﺎ ﮔﺴﺘﺮه­ وﺳﯿﻌﯽ از ﺗﺮﮐﯿﺐ و ﺷﮑﻞ امکان­پذیر می­ سازد. افزون بر این، ﺑﺎ اﺳﺘﻔﺎده از اﻧﻮاع ﻣﻮﻟﮑﻮل­ﻫﺎی ­زﯾﺴﺘﯽ و عامل­ های رﺷﺪ ﺑﻪﻫﻤﺮاه اﯾﻦ اﻟﯿﺎف، ﻣﯽ­ﺗﻮان ﭘﺎﺳﺦ ﺳﻠﻮﻟﯽ و ﺑﺎزدﻫﯽ درﻣﺎﻧﯽ را در فرایندﻫﺎی ﻣﻬﻨﺪﺳﯽ ﺑﺎﻓﺖ ارﺗﻘﺎ داد. همچنین، با انتخاب روش مناسب برای بارگذاری این مولکول­ ها، فرایند را در بافت مدنظر به ­خوبی کنترل و هدایت کرد. اﮔﺮﭼﻪ اﻧﺘﻘﺎل و رﻫﺎﯾﺶ ﮐﻨﺘﺮل­ﺷﺪه­ عامل­ های رشد ﻫﻨﻮز با ﭼﺎﻟﺶ­ﻫﺎی بسیاری مواجه است، اﻣﺎ اﻟﯿﺎف اﻟﮑﺘﺮورﯾﺴﯽ ­ﺷﺪه ﻣﯽ­ﺗﻮاﻧﻨﺪ روزﻧﻪ­ ﭘﯿﺸﺮﻓتی برای اﻧﺘﻘﺎل ﻫﺪﻓﻤﻨﺪ و ﮐﻨﺘﺮل­شده داروﻫﺎ و عامل ­های رﺷﺪ در ﻣﻬﻨﺪﺳﯽ ﺑﺎﻓﺖ ﺑﺎﺷﻨﺪ که ﺑﻪ ﻋﻨﻮان دارﺑﺴﺖ­ﻫﺎی رﻫﺎﯾﺶ در ﻋﻠﻮم ﭘﺰﺷﮑﯽ و ﻣﻬﻨﺪﺳﯽ، اﺳﺘﻔﺎده شوند.

کلیدواژه‌ها

عنوان مقاله [English]

Application of Electrospun Nanofibers in Tissue Engineering: Scaffolds with Sustained Release of Growth Factors

نویسندگان [English]

  • Fariba Ganji 1
  • Mahya Baradaran 1
  • ameneh seddighian 2
1 Tarbiat Modares University
2 Tarbiat Modares University
چکیده [English]

Tissue engineering is a novel approach in treatment, in which, three important factors of cell, molecule signals, and scaffolds play a vital role. Nowadays, tissue engineering scientists use nanofibrous scaffolds to treat different diseases, regenerate and replace different body tissues, so as to simulate native tissues, as well as making progress and development in nano fibrous scaffolds engineering studies. To conduct the aforementioned studies, electrospinning is preferred out of various routs, due to its simplicity and cost-effectiveness in fabrication of structures that make the production of micro/nanofibers with a wide range of compositions and forms feasible. Moreover, using various types of biomolecules, biodrugs, and growth factors that are incorporated with in these fibers, would make it possible to improve cell response and treatment yield in tissue engineering processes, along with a suitable control and conduction of the process by selecting a suitable method of molecule loading. Although, there are many challenges regarding loading and controlled delivery of biomolecules through electrospun micro/nanofibers, but they have the potential ability to enhance the drug and growth factor delivery for tissue engineering and drug therapy. In addition, these micro/nanofibers can also be utilized as releasing scaffolds in engineering and medical science.

کلیدواژه‌ها [English]

  • Electrospinning
  • Tissue Engineering
  • Controlled Delivery
  • Scaffold
  • Growth Factor

مراجع

  1. Xue J., Wu T., Dai Y., and Xia Y., Electrospinning and Electrospun Nanofibers: Methods, Materials, and Applications, Chem. Rev., 119, 5298–5415, 2019.
  2. Sheikholeslami Kandelousi P., Polycaprolactone-Based Electrospun Composites in Bone Tissue Engineering, Polymerization (Persian), 8, 26-35, 2018.
  3. Mashayekhi M.S. and Daemi H., MeltElectrospinning:An Overview on History, Methodes and Applications, Polymerization (Persian), 6, 107-115, 2015.
  4. Asadi Korayem M., and Daemi H., Alginate Electrospinning: Challenges and Solutions, Polymerization (Persian), 9, 33-43, 2019.
  5. Ghanian M.H., Barzin J., Zandi M., Ehsani M., and Kazemi-Ashtiani M., Controlling the Orientation of Electrospun Nanofibers by Conductive-Insulating Hybrid Collectors, Polymerization (Persian), 2, 51-58, 2014.
  6. Zhang Z., Hu J., and Ma P. X., Nanofiber-Based Delivery of Bioactive Agents and Stem Cells to Bone Sites, Adv. Drug Deliv. Rev., 64, 1129-1141, 2012.
  7. Ji W., Sun Y., Yang F., Van den Beucken J.J., Fan M., Chen Z., and Jansen J.A., Bioactive Electrospun Scaffolds Delivering Growth Factors and Genes for Tissue Engineering Applications, Pharmaceut. Res., 28, 1259-1272, 2011.
  8. Nie H., Soh B.W., Fu Y.C., and Wang C.H., Three‐Dimensional Fibrous PLGA/HAp Composite Scaffold for BMP‐2 Delivery, Biotech. Bioeng., 99, 223-234, 2008.
  9. Choi J.S., Leong K.W., and Yoo H.S., In Vivo Wound Healing of Diabetic Ulcers Using Electrospun Nanofibers Immobilized with Human Epidermal Growth Factor (EGF), Biomaterials, 29, 587-596, 2008.
  10. Aguirre-Chagala Y.E., Altuzar V., León-Sarabia E., Tinoco-Magaña J.C., Yañez-Limón J.M., and Mendoza-Barrera C., Physicochemical Properties of Polycaprolactone/ Collagen/Elastin Nanofibers Fabricated by Electrospinning, Mater. Sci. Eng. C, 76, 897-907, 2017.
  11. Deliormanl A.M., and Konyal R., Bioactive Glass/Hydroxyapatite-Containing Electrospun Poly(ε-caprolactone) Composite Nanofibers for Bone Tissue Engineering, J. Aust. Ceram. Soc., 55, 247-256, 2019.
  12. Zhu H., Yu D., Zhou Y., Wang C., Gao M., Jiang H., and Wang H., Biological Activity of a Nanofibrous Barrier Membrane Containing Bone Morphogenetic Protein Formed by Core‐Shell Electrospinning as a Sustained Delivery Vehicle, J. Biomed. Mater. Res. B: Appl. Biomater., 101, 541-552, 2013.
  13. Ji W., Yang F., Van den Beucken J. J., Bian Z., Fan M., Chen Z., and Jansen J.A., Fibrous Scaffolds Loaded with Protein Prepared by Blend or Coaxial Electrospinning, Acta Biomater., 6, 4199-4207, 2010.
  14. Jahanmardi Y., Tavanaie M.A., and Bagha A.R.T., Drug Delivery of Nanofibers Based on Biodegradable Synthetic Polymer Blends: A Review, Polymerization (Persian), 7, 13-29, 2016.
  15. Liu J.J., Wang C.Y., Wang J.G., Ruan H.J., and Fan C.Y., Peripheral Nerve Regeneration Using Composite Poly(lactic acid‐caprolactone)/Nerve Growth Factor Conduits Prepared by Coaxial Electrospinning, J. Biomed. Mater. Res. A, 96, 13-20, 2011.
  16. Haghighat F., Ravandi S.A.H., Esfahany M.N., and Valipouri A., A Comprehensive Study on Optimizing and Thermoregulating Properties of Core–Shell Fibrous Structures through Coaxial Electrospinning, J. Mater. Sci., 53, 4665-4682, 2018.
  17. Nie J., Wang Z.L., Li J.F., Gong Y., Sun J.X., and Yang S.G., Interface Hydrogen-Bonded Core-Shell Nanofibers by Coaxial Electrospinning, Chin. J. Polym. Sci., 35, 1001-1008, 2017.
  18. Lee J.S., Kim S.K., Jung B.J., Choi S.B., Choi E.Y., and Kim C.S., Enhancing Proliferation and Optimizing the Culture Condition for Human Bone Marrow Stromal Cells Using Hypoxia and Fibroblast Growth Factor-2, Stem Cell. Res., 28, 87-95, 2018.
  19. Hu X., Xiong Q., Xu Y., Zhang X., Pan X., Ma X., and Jia W., Association of Serum Fibroblast Growth Factor 19 Levels with Visceral Fat Accumulation is Independent of Glucose Tolerance Status, Nutr. Metab. Cardiovasc. Dis., 28, 119-125, 2018.
  20. Zhang H., Jia X., Han F., Zhao J., Zhao Y., Fan Y., and Yuan X., Dual-Delivery of VEGF and PDGF by Double-Layered Electrospun Membranes for Blood Vessel Regeneration, Biomaterials, 34, 2202-2212, 2013.
  21. Jin G., Lee S., Kim S.H., Kim M., and Jang J.H., Bicomponent Electrospinning to Fabricate Three-Dimensional Hydrogel-Hybrid Nanofibrous Scaffolds with Spatial Fiber Tortuosity, Biomed. Microdevices, 16, 793-804, 2014.
  22. Wang C., Tong S.N., Tse Y.H., and Wang M., Conventional Electrospinning vs. Emulsion Electrospinning: A Comparative Study on the Development of Nanofibrous Drug/Biomolecule Delivery Vehicles, Adv. Mater. Res., 410, 118-121, 2012.
  23. Briggs T., and Arinzeh T.L., Examining the Formulation of Emulsion Electrospinning for Improving the Release of Bioactive Proteins from Electrospun Fibers, J. Biom. Mater. Res. A, 102, 674-684, 2014.
  24. Yang Y., Xia T., Zhi W., Wei L., Weng J., Zhang C., and Li X., Promotion of Skin Regeneration in Diabetic Rats by Electrospun Core-Sheath Fibers Loaded with Basic Fibroblast Growth Factor, Biomaterials, 32, 4243-4254, 2011.
  25. Li B., Liu C., Zhou F., Mao X., and Sun R., Preparation of Electrospun Core–Sheath Yarn with Enhanced Bioproperties for Biomedical Materials, Biotechnol. Lett., 40, 279-284, 2018.
  26. Ionescu L.C., Lee G.C., Sennett B.J., Burdick J.A., and Mauck R.L., An Anisotropic Nanofiber/Microsphere Composite with Controlled Release of Biomolecules for Fibrous Tissue Engineering, Biomaterials, 31, 4113-4120, 2010.
  27. Gaharwar A.K., Mihaila S.M., Kulkarni A.A., Patel A., Di Luca A., Reis R.L., and Khademhosseini A., Amphiphilic Beads as Depots for Sustained Drug Release Integrated into Fibrillar Scaffolds, J. Controll. Release, 187, 66-73, 2014.
  28. Omidvar N., Ganji F., and Baghban-Eslaminejad M.R., In vitro Osteogenic Induction of Human Marrow-Derived Mesenchymal Stem Cells by PCL fibrous Scaffolds Containing Dexamethazone-Loaded Chitosan Microspheres, J. Biomed. Mater. Res. A, 1-11, 2016.
  29. Rajzer I., Menaszek E., Kwiatkowski R., Planell J.A., and Castano O., Electrospun Gelatin/Poly(ε-caprolactone) Fibrous Scaffold Modified with Calcium Phosphate for Bone Tissue Engineering, Mater. Sci. Eng. C, 44, 183-190, 2014.
  30. Li L., Zhou G., Wang Y., Yang G., Ding S., and Zhou S., Controlled Dual Delivery of BMP-2 and Dexamethasone by Nanoparticle-Embedded Electrospun Nanofibers for the Efficient Repair of Critical-Sized Rat Calvarial Defect, Biomaterials, 37, 218-229, 2015.

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