کامپوزیت‌های الکتروریسی‌شده بر پایه پلی‌کاپرولاکتون در مهندسی بافت استخوان

نوع مقاله: تالیفی

نویسنده

دانشگاه صنعتی بابل، بابل، ایران

چکیده

بافت استخوان طبیعی در اثر شکستگی‌ها و پیری دچار نقص می‌شود. با توجه به روند خودترمیمی طولانی‌مدت یا بازسازی‌نشدن در آسیب‌های شدید، موضوع مهندسی بافت مطرح شده است. مهم‌ترین عوامل موفقیت در مهندسی بافت، به‌کارگیری سلول و داربست مناسب است. بدین منظور، سلول‌های متنوعی همچون استخوان‌سازها و سلول‌های بنیادی جنینی و مزانشیمی استفاده می‌شوند. ولی ویژگی‌های منحصربه‌فرد سلول‌های مزانشیمی موجب شد تا کاربرد گسترده‌ای در حوزه مهندسی بافت پیدا کنند. فنون مختلفی مانند الکتروریسی نیز برای ساخت داربست وجود دارد. روش الکتروریسی به‌دلیل شباهت نانوالیاف با ماتریس خارج سلولی بافت طبیعی، قابلیت انتخاب مواد مختلف، نسبت سطح به حجم زیاد الیاف و بازسازی هرچه بیشتر بافت نسبت به سایر روش‌ها مورد توجه قرارگرفته‌است. پلی‌کاپرولاکتون زیست‌پلیمری سنتزی است که به‌طور گسترده در کاربردهای پزشکی استفاده می‌شود. مهم‌ترین مزایای استفاده از این نوع پلیمر، استحکام مکانیکی زیاد، فراورش‌پذیری ساده، سمیت کم و تحریک ضعیف سامانه ایمنی است. به‌طور عمده پلیمرها را به‌منظور دست‌یابی به خواص مکانیکی مطلوب با سرامیک‌ها کامپوزیت می‌کنند. داربست‌های مهندسی بافت استخوان باید زیست‌سازگار، زیست‌تخریب‌پذیر، مستحکم، متخلخل، دارای اندازه تخلخل میکرومتری و از نظر شکل‌شناسی، صاف، یکنواخت و بدون گره باشند. همچنین، زبری سطحی و آب‌دوست بودن غشا سبب آسان‌شدن رفتارهای سلولی می‌شود. هدف از این پژوهش، ارزیابی و مشخصه‌یابی داربست‌های الکتروریسی‌شده بر پایه پلی‌کاپرولاکتون در بازسازی و ترمیم بافت استخوان بوده‌است.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Polycaprolactone-based Electrospun Composites in Bone Tissue Engineering

نویسنده [English]

  • peyman sheikholeslami kandelousi
Babol University of Technology, Babol, Iran
چکیده [English]

Natural bone tissue defects are caused by fractures and aging. The subject of tissue engineering has been raised and developed due to long-term self-healing processes or the lack of regeneration in severe injuries. The most important factors in successful tissue engineering are the selection of suitable cells and scaffolds. Various cells such as osteoblast, embryonic and mesenchymal stem cells are used, but the unique properties of mesenchymal cells have led to extensive application in the tissue engineering. Electrospinning is the one among several techniques to obtain scaffolds. In electrospinning method, because of the similarity of nanofibers with the extracellular matrix of the native tissue, the choice of different materials, the high surface area/volume ratio and the reconstruction of the tissue more than other methods have been considered. Polycaprolactone is a synthetic biopolymer that is widely employed in medical applications. The most important advantages of this polymer are high mechanical strength, simple processability, low toxicity, low immunogenicity. Mainly, the polymers are combined with ceramics to achieve the desired mechanical properties. Bone tissue engineering scaffolds should be biocompatible, biodegradable, high strength, porous, micro-scale pore size, and have smooth, uniform and free-bead morphology. Surface roughness and hydrophilicity of a membrane facilitates cellular behavior. The purpose of this research was the characterization and evaluation of polyacroplactone-based electrospinning scaffolds for bone tissue repair.

کلیدواژه‌ها [English]

  • electrospinning
  • scaffold
  • polycaprolactone
  • composite
  • cell
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